Journal: Cell Death Discovery

Article Title: Interleukin-1β induces and accelerates human endometrial stromal cell senescence and impairs decidualization via the c-Jun N-terminal kinase pathway

doi: 10.1038/s41420-024-02048-6

Figure Lengend Snippet: Antibody table.

Article Snippet: SignalSilence SAPK/JNK siRNAII , Cell Signaling Technology, 6233 , 100 nM , Transfection , , .

Techniques: Transfection

Journal: Scientific Reports

Article Title: Autotetraploid cell Line induced by SP600125 from crucian carp and its developmental potentiality

doi: 10.1038/srep21814

Figure Lengend Snippet: ( A ) Western blot analysis detected Jnk (Jnk1/Jnk2) expression in SP600125-induced tetraploid cells and normal diploid cells. Error bars represent the means ± SD; n = 3. P < 0.001. ( B ) shRNA knockdown of jnk1/jnk3 . Cells transfected with an empty vector were used as control. Knockdown was evaluated by Q-PCR. Expression values are relative to actin expression on set as 1. Error bars represent the means ± SD; n = 3. P < 0.001. ( C ) FACS analysis of jnk1 -shRNA (blue) and jnk3 -shRNA (green) transfected cell DNA content. An empty vector (red) was transfected as the control.

Article Snippet: Jnk shRNA lentiviral vector plasmids (shRNA jnk1 Plasmid (m): sc-29381-SH; and shRNA jnk3 Plasmid (m): sc-39104) was purchased from Santa Cruz (UK) and the three shRNAs were pooled.

Techniques: Western Blot, Expressing, shRNA, Knockdown, Transfection, Plasmid Preparation, Control

Journal: Cancer letters

Article Title: Carcinogenic metalloid arsenic induces expression of mdig oncogene through JNK and STAT3 activation

doi: 10.1016/j.canlet.2014.01.002

Figure Lengend Snippet: Silencing STAT3 and JNK repressed mdig expression. A. BEAS-2B cells were transfected with 50 nM control siRNA (siCtrl), or STAT3 specific siRNA (siSTAT3) and cultured for 24 h. Cells were cultured in the presence of 20 μM As3+ for an additional 2 h. The levels of STAT3 activation, mdig protein, JNK and Akt activation were determined by Westernblotting. B. Silencing JNK2, but not JNK1, reduced the levels of basal and As3+-induced mdig. Cell transfection with siRNA and treatment are the same as in A.

Article Snippet: SiRNA that specifically silences JNK1 was purchased from Eurofins (Huntsville, AL); control siRNA and siRNAs specifically silencing the JNK2 and STAT3 were purchased from Santa Cruz Technology (Santa Cruz, CA).

Techniques: Expressing, Transfection, Control, Cell Culture, Activation Assay